Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NELFE

Cell type

Cell type Class
Blood
Cell type
MOLM-13
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MOLM-13
vector
HA-FKBP-hSETD1A-puro;Cas9-Blast;SETD1A Ex8 sgRNA-GFP
chip antibody
NELFe (#ab170104, Abcam)
stimulation
DMSO
time
24h

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, cells were fixed with 2mM DSG and 1% formaldehyde, and samples from sonicated nuclear fraction were incubated with each antibody and histone-DNA complexes were isolated. ChIP-seq libraries were prepared using NEBNext ChIP-Seq Library Preparation Set for Illumina (New England Biolabs) following the manufacturer's protocol.Libraries for RNA-seq were prepared using the TruSeq Stranded mRNA Sample Prep Kit (Illumina).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
24837490
Reads aligned (%)
97.3
Duplicates removed (%)
7.0
Number of peaks
17647 (qval < 1E-05)

hg19

Number of total reads
24837490
Reads aligned (%)
96.7
Duplicates removed (%)
8.0
Number of peaks
17581 (qval < 1E-05)

Base call quality data from DBCLS SRA